Anti-Mouse CD4 (Clone GK1.5) - DyLight 550

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Product Concentration

0.2 mg/ml

Formulation

This DyLight® 550 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight® 550 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

Excitation Laser

Yellow Laser (563 nm)

RRID

AB_2828692

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Additional Reported Applications For Relevant Conjugates ?

B
IP
Depletion
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CD4 (CD4) (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.

Background

CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.¹

Antigen Details

Protein

CD4

Ligand/Receptor

MHC class II molecule

PubMed

CD4

NCBI Gene Bank ID

12504

UniProt.org

Information on Uniprot.org

References & Citations

1. Hendrickson WA et al. (1994) Structure 2: 59

Technical Protocols