Anti-Mouse CTLA-4 (Clone 9H10) - Purified In vivo GOLD™ Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Syrian Hamster

Immunogen

Mouse CTLA-4-human IgG1 fusion protein

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2737453

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone 9H10 recognizes an epitope on mouse CTLA-4.

Antigen Distribution

CTLA-4 is expressed on activated T and B lymphocytes.

Background

CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity.

Antigen Details

Protein

CTLA-4

Ligand/Receptor

CD80 (B7-1), CD86 (B7-2)

Function

Negative regulator of T cell activation

PubMed

CTLA-4

NCBI Gene Bank ID

12477

UniProt.org

Information on Uniprot.org

References & Citations

1.) Wurster S. et al. (2020) The Journal of Infectious Diseases 222(6):1989–994 Journal Link

Technical Protocols