Antibody Details
Product Details
Reactivity Species
Chicken
Host Species
Donkey
Immunogen
Purified Chicken IgY/IgG (H& L) (> 98%)
Product Concentration
0.5 - 1.0 mg/ml
Formulation
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This secondary antibody conjugate should be stored desiccated at 2-8°C.
Expiration Date
One year from date of receipt
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
It is recommended that working dilutions be prepared fresh daily. FC: 1:100 - 1:800IF: 1:100 - 1:800Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
RRID
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Donkey F(ab')2 Anti-Chicken IgY/IgG (H& L) recognizes Chicken IgY/IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Antigen Details
Protein
IgY/IgG (H& L)
PubMed
IgY/IgG (H& L)