Anti-Human CD14 - Purified

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Product Concentration

0.5 mg/ml

Formulation

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This purified antibody is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2828600

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this UCHM-1 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

IHC (Frozen)

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone UCHM-1 recognizes an epitope on human CD14.

Antigen Distribution

CD14 is expressed on monocytes, macrophages, dendritic cells, and granulocytes.

Background

Cluster of differentiation 14 also known as CD14 is a cell surface glycoprotein¹ expressed in monocytes, macrophages, polymorphonuclear neutrophils, chondrocytes², B-cells³, dendritic cells.⁴ CD14 functions to enhance activation of cells by lipopolysaccharide (LPS).⁵

Antigen Details

Protein

CD14

Ligand/Receptor

LPS

PubMed

CD14

NCBI Gene Bank ID

929

UniProt.org

Information on Uniprot.org

Research Area

Cell Biology

.

Immunology

.

Neuroinflammation

.

Neuroscience

References & Citations

1. Ulrich Costabel et al. (1995) CHEST 108: 1614
2. Ulevitch RJ et al. (1993) Immunobiology 187: 227
3. Schumann RR et al. (1994) Medical Microbiol Immunol. 183: 279
4. Verhasselt V et al. (1997) J Immunol. 158: 2919
5. Kirkland TN et al. (2000) J Biol Chem. 275: 3144

Technical Protocols