Anti-Human CD6 (Clone 123119)

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

NS0-Derived Recombinant Human CD6 Extracellular Domain (Accession # CAA43306)

Endotoxin Level

< 0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2828508

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Human CD6 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human CD6 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Cluster of Differentiation 6 (CD6) (Clone 123119) recognizes an epitope on Human CD6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

CD6 (Cluster of Differentiation 6) is a cell surface glycoprotein that is involved in T cell activation¹ and a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF).² CD6 can deliver coactivating signals to mature T lymphocytes and regulate its antigen specific and autoreactive responses.³

Antigen Details

Protein

CD6

PubMed

CD6

NCBI Gene Bank ID

923

UniProt.org

Information on Uniprot.org

References & Citations

1. Parnes, JR. et al. (1995) Eur J Immunol. 25: 2765

2. Bajorath, J. et al. (1997) Biochemistry 36: 2637

3. Fox, DA. et al. (1996) Immunology 88: 537

Technical Protocols