Antibody Details
Product Details
Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this SJ5-1B4 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Mouse Anti-Human CD10 (Clone SJ5-1B4) recognizes Human CD10. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD10 (Anti-CALLA) recognizes a human common acute lymphoblastic leukemia antigen. CD10 is a (Mr 100 kDa) surface glycoprotein with associated neutral endopeptidase activity.1, 2
Antigen Distribution
The CD10 antigen is found on cells from subjects with acute B-lymphoid leukemia.3 The antigen is also expressed in various degrees on progenitor B-cells, renal epithelium, fibroblasts, granulocytes, early T-lymphocytes and germinal center cells.
Background
Anti-Human CD10 may be used in studies characterizing non-T-cell common acute lymphoblastic leukemias or differentiation of early lymphocyte lineages.
Antigen Details
Protein
CD10
Ligand/Receptor
Biologically active peptides including opioid enkephalins, bradykinin, angiotensions I & II
PubMed
CD10
NCBI Gene Bank ID
4311
UniProt.org
Information on Uniprot.org
Research Area
Immunology
References & Citations
1. Leukocyte Typing V (1995) Oxford University Press2. Billings, R. et al. (1978) J. Natl. Cancer Inst. 61:5833. Ritz, J. et al. (1980) Nature 283:5834. Foon, K. A. et al. (1982) Blood 60:1
Technical Protocols