Donkey F(ab’)2 Anti-Chicken IgY/IgG (H&L)-HRP

Antibody Details

Product Details

Reactivity Species

Chicken

Host Species

Donkey

Immunogen

Purified Chicken IgY/IgG (H& L) (> 98%)

Product Concentration

0.8 mg/ml

Formulation

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

Product Preparation

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Storage and Handling

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. Caution: This horseradish peroxidase conjugate does not contain preservatives. To avoid microbial contamination, store the reconstituted conjugate containing glycerol at -20°C in a manual defrost freezer.

Expiration Date

One year from date of reconstitution

Country of Origin

USA

Shipping

Next Day Ambient

Working Concentration

It is recommended that working dilutions be prepared fresh daily. WB: 1:5, 000 - 1:100, 000WB Chemiluminescent: 1:10, 000 - 1:200, 000ELISA: 1:5, 000 - 1:100, 000IHC: 1:500 - 1:5, 000Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

RRID

AB_2893549

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Donkey F(ab')₂ Anti-Chicken IgY/IgG (H& L) recognizes Chicken IgY/IgG (H& L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein

IgY/IgG (H& L)

PubMed

IgY/IgG (H& L)