Anti-Human CD5 (Clone UCHT-2) - Purified In vivo Functional GOLD™ Functional Grade

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day Ambient

RRID

AB_2893537

Applications and Recommended Usage
Quality Tested by Leinco

FC The suggested concentration for this UCHT-2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Additional Reported Applications For Relevant Conjugates

IHC (Frozen)
WB
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human CD5 (Clone UCHT-2) recognizes Human CD5. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD5 recognizes a human T-lymphocyte surface membrane antigen (Mr 67 kDa).

Antigen Distribution

The CD5 antigen is present on approximately 70% of normal peripheral blood lymphocytes and > 90% of all T-lymphocytes. T-lymphocytes in the thymus stain weakly while peripheral T-cells stain strongly. Monocytes, granulocytes and NK cells are not stained.¹ Anti-CD5 also reacts with a distinct subset of normal B-lymphocytes, occasional cells in B-lymphocyte areas of spleen and lymph node, and most Ig+ B-chronic lymphoblastic leukemia (CLL) cells. The CD5 antigen has also been shown to be present on some lymphomas.

Background

Anti-Human CD5 can be used in research to study B-lymphocyte subsets or characterization of leukemias and lymphomas.

Antigen Details

Protein

CD5

Ligand/Receptor

CD72

PubMed

CD5

NCBI Gene Bank ID

921

UniProt.org

Information on Uniprot.org

Research Area

Costimulatory Molecules

.

Immunology

References & Citations

1. Leukocyte Typing VI (1995) Oxford University Press
2. Leukocyte Typing III (1987) Oxford University Press
3. Beverly, P. C. L. et al. (1981) "Protides of the Biological Fluids, " Vol. XXIX, Peeters, H. (ed.), Pergamon Press, Oxford, 653

Technical Protocols