ArtNr |
ICH1193-25mg |
Hersteller |
IchorBio
|
Menge |
25mg |
Kategorie |
|
Typ |
Antibody Monoclonal |
Applikationen |
FC, ELISA, FA |
Clon |
LO-CD2a |
Specific against |
Human |
Host |
Rat |
Isotype |
IgG2b Kappa |
Purity |
>95% by SDS-PAGE |
Formula |
Sterile, preservative-free, solution in PBS. BSA and Azide free. |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
BTI-322, CD2, CD2 molecule, T11, SRBC, LFA-2, T-cell surface antigen CD2, LFA-3 receptor, rosette receptor, erythrocyte receptor, lymphocyte-function antigen-2, T-cell surface antigen T11/Leu-5, CD2 antigen (p50), sheep red blood cell receptor |
Lieferbar |
|
Specificity |
Anti-CD2 antibody (clone LO-CD2a) activity is directed against human CD2. |
Manufacturers Product Category |
Low Endotoxin, anti-mouse |
Isotype Information |
Rat IgG2b Kappa |
Purification method |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Antigen Distribution |
CD2 is expressed on all mature human T cells, most thymocytes, NK cells and a small proportion of bone marrow cells. |
Background |
LO-CD2a strongly inhibits mixed lymphocyte culture (MLC) 3 and induces T cell apoptosis. Additionally, LO-CD2a does not induce proliferation of resting T cells, and inhibits T cell proliferation induced by OKT3 antibody, induces very low levels of cytokine release, and also inhibits cytokine production induced by OKT3, LO-CD2a strongly inhibits proliferation of peripheral blood mononuclear cells stimulated with soluble OKT3, and induces human T cell hyporesponsiveness. Adding LO-CD2a to a primary MLC inhibits the T cell proliferation response and has a subsequent inhibitory effect on the secondary immune response, thereby blocking primary and memory alloantigen proliferative responses. The human T cell hyporesponsiveness in the secondary MLC is restricted to alloantigens and occurs both in the absence of antibody and after cell surface re-expression of CD2 |
Immunogen |
LO-CD2a was developed as an immunosuppressive agent for use in organ allotransplantation. LO-CD2a was produced by immunizing a LOU/C rat with purified human T lymphocytes and fusing the splenocytes with the non-secreting rat fusion cell line IR983F, resulting in a rat IgG2b mAb of the IgK-1a allotype |
Concentration |
≥ 2.0 mg/ml |
Formulation |
Sterile, preservative-free, solution in PBS. BSA and Azide free. |
Endotoxin |
<1.0 EU/mg as determined by the LAL method |
Aggregation |
Aggregation level ≤ 5% |
Storage |
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Application Notes |
Each investigator should determine their own optimal working dilution for specific applications. |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.