ArtNr |
ICH1163-5MG |
Hersteller |
IchorBio
|
Menge |
5mg |
Kategorie |
|
Typ |
Antibody |
Applikationen |
WB, FC, IP, ELISA |
Clon |
7G7B6 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG2a |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
IL2RA, Interleukin-2 receptor subunit alpha, TAC, p55 |
Lieferbar |
|
Specificity |
Clone 7G7B6 reacts with human CD25 |
Manufacturers Product Category |
Low Endotoxin, anti-human |
Isotype Information |
Mouse IgG2a |
Purification method |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Antigen Distribution |
IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes |
Background |
CD25 (IL-2Rα) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2Rα is a transmembrane glycoprotein comprised of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. IL-2Rα heterodimerizes with IL-2Rβ (CD122) and IL-2Rγ (CD132) and forms the IL-2 receptor complex. IL-2Rα by itself binds IL-2 with relatively low affinity, while the IL-2 receptor complex binds IL-2 with high affinity. IL-2Rα mutations cause severe immunodeficiency. |
Immunogen |
Recombinant human peripheral blood mononuclear cells (PBMCs) |
Concentration |
1mg/ml |
Formulation |
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Aggregation |
Aggregation level ≤ 5% |
Storage |
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Application Notes |
Each investigator should determine their own optimal working dilution for specific applications. |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.