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Anti-PD-1 (RMP1-14) - Ultra-Low Endotoxin Europäischer Partner

ArtNr ICH1132UL-25MG
Hersteller IchorBio
Menge 25 mg
Quantity options 100 mg 25 mg 50 mg 5 mg
Kategorie
Typ Antibody
Applikationen WB, FC, Blocking, FA
Clon RMP1-14
Specific against Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2a Kappa
Purity >98% by SDS-PAGE and HPLC
Citations Chen et al. A magneto-activated nanoscale cytometry platform for molecular profiling of small extracellular vesicles. Nature Communications
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Programmed cell death protein 1, Pdcd1, CD279
Versandbedingung Gekühlt
Lieferbar
Specificity Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1
Manufacturer - Applications
Western Blot, Blocking, Flow Cytometry, Functional Assays
Manufacturer - Category
Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG2a Kappa
Manufacturer - Targets
PD-1
Shipping Temperature
Blue ice
Storage Conditions
anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 1%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
Subset of double negative thymocytes, activated T and B cells
Application Notes
Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required.
Each investigator should determine their own optimal working dilution for specific applications.
Background
CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.
Concentration
≥ 2.0 mg/ml
Endotoxin
≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
Mouse PD-1 transfected BHK cells
IMPACT Pathogen Test
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
Related Antibodies
Isotype Control
Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL)
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25 mg
Lieferbar: In stock
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