ArtNr |
ICH1132-25MG |
Hersteller |
IchorBio
|
Menge |
25 mg |
Quantity options |
100 mg
1 mg
25 mg
50 mg
5 mg
|
Kategorie |
|
Typ |
Antibody |
Applikationen |
WB, FC, Blocking, FA |
Clon |
RMP1-14 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Rat |
Isotype |
IgG2a Kappa |
Purity |
>95% by SDS-PAGE and HPLC |
Citations |
Uncoupling immune trajectories of response and adverse events from anti-PD-1 immunotherapy in hepatocellular carcinoma Rational design of synthetically tractable HDAC6/HSP90 dual inhibitors to destroy immune-suppressive tumor microenvironment PD-1 Blockade Restores the Proliferation of Peripheral Blood Lymphocyte and Inhibits Lymphocyte Apoptosis in a BALB/c Mouse Model of CP BVDV Acute Infection Kauffman et al. PSGL-1 Blockade Induces Classical Activation of Human Tumor-Associated Macrophages. Cancer Research Communications 2023 Liang et al. SIX4 Controls STING Expression Enhancing anti-PD-1 Efficacy. bioRxiv Xu et al. One-carbon unit supplementation fuels tumor-infiltrating T cells and augments checkpoint blockade. bioRxiv Benguigui et al. Interferon-stimulated neutrophils as a predictor of immunotherapy response. 2024, Cancer Cell 42, 253–265 |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Programmed cell death protein 1, Pdcd1, CD279 |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Specificity |
Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1 |
Manufacturer - Applications |
Western Blot, Blocking, Flow Cytometry, Functional Assays |
Manufacturer - Category |
Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Rat IgG2a Kappa |
Manufacturer - Targets |
PD-1 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
Subset of double negative thymocytes, activated T and B cells |
Application Notes |
Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production. |
Concentration |
1.0 - 5.0 mg/ml |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1 |
Purification method |
This monoclonal antibody was purified using Protein G |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
Mouse PD-1 transfected BHK cells |
Related Antibodies |
|
Isotype Control |
Rat IgG2a In Vivo Isotype Control - Low Endotoxin [1-1] (ICH2244) |
Buffer |
ICH3001-100ml ICH3002-100ml ICH3003-100ml |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.