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Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Ultra-Low Endotoxin Europäischer Partner

ArtNr ICH1091UL-25MG
Hersteller IchorBio
Menge 25 mg
Quantity options 100 mg 25 mg 50 mg 5 mg
Kategorie
Typ Antibody
Applikationen WB, FC, IHC, Blocking
Clon 29F.1A12
Specific against Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2a Kappa
Purity >98% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Programmed cell death protein 1, Pdcd1, CD279
Versandbedingung Gekühlt
Lieferbar
Specificity Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
Manufacturer - Applications
Flow Cytometry, Western Blot, Blocking, IHC (Frozen)
Manufacturer - Category
Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG2a Kappa
Manufacturer - Targets
PD-1
Shipping Temperature
Blue ice
Storage Conditions
anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 1%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells
Application Notes
Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml.
Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3, 6, 9 @ 200 ug per animal per injection) then the tumor will regress, and disappear.
Each investigator should determine their own optimal working dilution for specific applications.
Background
Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
Concentration
≥ 2.0 mg/ml
Endotoxin
≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
PD-1 cDNA followed by PD-1-Ig fusion protein
IMPACT Pathogen Test
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
Related Antibodies
Isotype Control
Rat IgG2a In Vivo Isotype Control - Ultra Low Endotoxin [1-1] (ICH2244UL)
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 25 mg
Lieferbar: In stock
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