Anti-Mouse PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12)

Low Endotoxin, anti-mouse

Rat IgG2a Kappa

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells

PD-1 cDNA followed by PD-1-Ig fusion protein

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Aggregation level ≤ 5%

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml. - Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3, 6, 9 @ 200 ug per animal per injection) then the tumor will regress, and disappear. - Each investigator should determine their own optimal working dilution for specific applications.