ArtNr |
ICH1056-50MG |
Hersteller |
IchorBio
|
Menge |
50 mg |
Quantity options |
100 mg
1 mg
25 mg
50 mg
5 mg
|
Kategorie |
|
Typ |
Antibody |
Applikationen |
WB, FC, IP, IHC, Blocking |
Clon |
2.4G2 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Rat |
Isotype |
IgG2b |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Low affinity immunoglobulin gamma Fc region receptor II, Fcgr2, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Ly-17, Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, FcRIII, Fc-gamma RIII |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Specificity |
Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors |
Manufacturer - Applications |
Western Blot, Blocking, Flow cytometry, IHC (Frozen), Immunoprecipitation, CODEX |
Manufacturer - Category |
Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Rat IgG2b |
Manufacturer - Targets |
CD32/CD16 |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 5% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes. |
Application Notes |
Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo. |
Concentration |
0.5 mg/ml |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors |
Purification method |
This monoclonal antibody was purified using Protein G |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Antibodies against the same target (Cat No.) |
|
Isotype Control |
Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243) |
Buffer |
ICH3001-100ml ICH3002-100ml ICH3003-100ml |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.