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  • Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin

Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin Europäischer Partner

ArtNr ICH1056-25MG
Hersteller IchorBio
Menge 25 mg
Quantity options 100 mg 1 mg 25 mg 50 mg 5 mg
Kategorie
Typ Antibody
Applikationen WB, FC, IP, IHC, Blocking
Clon 2.4G2
Specific against Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2b
Purity >95% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Low affinity immunoglobulin gamma Fc region receptor II, Fcgr2, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Ly-17, Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, FcRIII, Fc-gamma RIII
Versandbedingung Gekühlt
Lieferbar
Specificity Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
Manufacturer - Applications
Western Blot, Blocking, Flow cytometry, IHC (Frozen), Immunoprecipitation, CODEX
Manufacturer - Category
Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG2b
Manufacturer - Targets
CD32/CD16
Shipping Temperature
Blue ice
Storage Conditions
anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 5%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes.
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Background
CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.
Concentration
0.5 mg/ml
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Antibodies against the same target (Cat No.)
ICH1010-1MG
Isotype Control
Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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Menge: 25 mg
Lieferbar: In stock
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