ArtNr |
ICH1041UL-25MG |
Hersteller |
IchorBio
|
Menge |
25 mg |
Quantity options |
100 mg
25 mg
50 mg
5 mg
|
Kategorie |
|
Typ |
Antibody |
Applikationen |
WB, FC, IP, IHC, Blocking, IV |
Clon |
145-2C11 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Hamster - Armenian |
Isotype |
IgG |
Purity |
>98% by SDS-PAGE and HPLC |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e |
Versandbedingung |
Gekühlt |
Lieferbar |
|
Specificity |
Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e |
Manufacturer - Applications |
IHC (Frozen), Flow Cytometry, Immunoprecipitation, Western Blot, In vivo Depletion, Blocking |
Manufacturer - Category |
Ultra-Low Endotoxin, anti-mouse, monoclonal antibody |
Manufacturer - Isotype |
Armenian Hamster IgG |
Manufacturer - Targets |
CD3e |
Shipping Temperature |
Blue ice |
Storage Conditions |
anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. |
Aggregation |
Aggregation level ≤ 1% |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Antigen Distribution |
The CD3 complex is present on thymocytes (differentiation dependent), mature T cells, NK-T cells of all mouse strains tested. |
Application Notes |
Blocking: The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD3e is a 20 kD transmembrane protein and is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
Concentration |
1.0 - 5.0 mg/ml |
Endotoxin |
≤ 0.5 EU/mg as determined by the LAL method |
Manufacturer - Specificity |
Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin (145-2C11) recognizes an epitope on Mouse CD3e |
Purification method |
This monoclonal antibody was purified using Protein A |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Immunogen |
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37 |
IMPACT Pathogen Test |
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our hamster antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Hamster parvovirus Lymphocytic choriomeningitis Minute virus of mice Theiler's murine encephalomyelitis virus Sendai virus Reovirus 3 Hantaan virus |
Related Antibodies |
|
Antibodies against the same target (Cat No.) |
|
Isotype Control |
Armenian Hamster IgG Isotype Control for In Vivo - Ultra Low Endotoxin [PIP] (ICH2251UL) |
Buffer |
ICH3001-100ml ICH3002-100ml ICH3003-100ml |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.