ArtNr |
ICH1010-1MG |
Hersteller |
IchorBio
|
Menge |
1mg |
Kategorie |
|
Typ |
Antibody |
Applikationen |
FC, IHC, Blocking |
Clon |
3G8 |
Specific against |
Human |
Host |
Mouse |
Isotype |
IgG1 Kappa |
Purity |
>95% by SDS-PAGE and HPLC |
ECLASS 5.1 |
32160702 |
ECLASS 6.1 |
32160702 |
ECLASS 8.0 |
32160702 |
ECLASS 9.0 |
32160702 |
ECLASS 10.0.1 |
32160702 |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
in vivo |
Lieferbar |
|
Specificity |
Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16 |
Aggregation |
Aggregation level <= 5% |
Antigen Distribution |
CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. |
Application Notes |
Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is <=2.0 ug per million cells in 100 ul volume. Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Each investigator should determine their own optimal working dilution for specific applications. |
Background |
CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. |
Concentration |
>= 2.0 mg/ml |
Endotoxin |
<= 1.0 EU/mg as determined by the LAL method |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added. |
Immunogen |
Human PMN cells |
Product Type |
Low Endotoxin, anti-human |
Purification method |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Storage |
anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8C. |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.