ArtNr |
BS90743-50 |
Hersteller |
Bioworld Technology, Inc
|
Menge |
50ul |
Kategorie |
|
Typ |
Antibody Primary |
Applikationen |
WB, IF, IHC, ICC |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
IRS-1, IRS1, HIRS 1, HIRS1, Insulin receptor substrate 1, IRS 1, IRS-1, IRS1, IRS1, OTTHUMP00000164234, |
Lieferbar |
|
Swiss-Prot |
P35568(Human) |
Application_all |
WB:1:1, 000-1:2, 000 ICC:1:50-1:200 IHC:1:50-1:200 |
Background |
The insulin receptor substrate-1 (IRS-1), a protein major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphotyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1. |
Product |
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
Purification And Purity |
ProA affinity purified |
Storage And Stability |
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles. |
Specificity |
IRS1 polyclonal antibody detects endogenous levels of IRS1 protein. |
BiowMW |
170 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Immunogen |
recombinant protein |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.