HRP Conjugated AffiniPure Rabbit Anti-Rat IgG (H+L)

This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all rabbit serum proteins, except the specific antibody for rat IgG.

Secondary antibodies offer increased versatility enabling users to use many detection systems such as HRP, AP and fluorescence. They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody, and secondary antibodies' FC regions provide further binding locations for biotin, enable the use of ABC and SABC to further amplify the signal. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species. They are then purified through immunoaffinity chromatography and modified with antibody fragmentation, label conjugation, etc., to generate highly specific reagents.

Diluted by neutral PBS or TBS. Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.

1mg/ml

Rat IgG (whole molecule).

At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.