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Monocyte Chemotactic Protein-1 (MCP-1/MCAF)

Monocyte Chemotactic Protein-1 (MCP-1/MCAF)

ArtNr	ANOG-MO-C40021B
Hersteller	Anogen
Menge	0.5mg
Kategorie	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Antibodies Western Blot ELISA Lipid & Fatty Acid Transport Primary Antibodies By Organ Heart & Blood Cancer Neutralization
Typ	Antibody Monoclonal
Format	Aff. Pur
Applikationen	WB, ELISA, NT
Clon	S14
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 kappa
Protein Familie	Cytokine, Growth Factors
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Similar products	MCP-1/MCAF
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Description	Mouse monoclonal antibody to human Monocyte Chemotactic Protein-1 (MCP-1)/ Monocyte Chemotactic and Activating Factor (MCAF)
Target Protein	Human MCP-1
Immunogen	Purified recombinant human MCP-1
Specificity	This antibody reacts with natural and recombinant human MCP-1.
Purification	Protein G affinity purified
Species Reactivity	Human, other species not tested
Cross Reactivity	This antibody does not react with human interleukin-8 (IL-8) and other human cytokines tested such as interleukin-1beta (IL-1beta), serum amyloid A (SAA) and epidermal growth factor (EGF).
Myeloma	Sp2/0-Ag14
Background	Monocyte chemotactic and activating factor (MCAF) is also called monocyte chemotactic protein-1 (MCP-1) and chemokine (C-C motif) ligand 2 (CCL2). It is primarily secreted by monocytes, macrophages and dendritic cells. This cytokine displays chemotactic activity for monocytes, T-cells, and basophils, but not for neutrophils or eosinophils. MCAF causes the degranulation of basophils and mast cells, and augments the activity of monocyte and macrophage. MCAF plays an important role in inflammation, angiogenesis, auto-immune diseases, renal diseases, chronic infection and granuloma formation.
Formulation	Lyophilized from a solution in 0.01M PBS, pH 7.2
Reconstitution	Double distilled water is recommended to adjust the final concentration to 1.00mg/mL.
Application info	ELISA: This antibody can be used as a capture antibody in sandwich ELISA for human MCP-1 detection in combination with a monoclonal tracer/detection antibody (HRP-conjugated, Cat. No.: MO-C40021T or Biotin-conjugated, Cat. No.: MO-C40021TB). Approximately 1.5pg/mL of MCP-1 in serum/plasma or 4pg/mL of MCP-1 in medium can be detected with an assay range of 0-1600pg/mL. Neutralizing: The antibody (clone S14) at a concentration of 0.5ug/mL, neutralized 1nM recombinant human MCP-1 induced monocyte chemotaxis in blind well chemotaxis chambers. It was also found that S-14 could inhibit the activity of native MCP-1 at concentrations similar to inhibitory doses for recombinant MCP-1. Western Blot: 0.1ug/lane of recombinant human MCP-1 can be detected when antibody is used at 0.1-1ug/mL.
Research Area	Cytokine, chemotaxis, inflammation
Storage	Store at -20C

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Menge: 0.5mg

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Lieferung vsl. bis 21.02.2025

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