FGFR2 Antibody (N-term)

Synthetic Peptide

FGFR2 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member is a high-affinity receptor for acidic, basic and/or keratinocyte growth factor, depending on the isoform. Mutations in the gene are associated with many craniosynostotic syndromes and bone malformations. The genomic organization of the gene encompasses 20 exons. Alternative splicing in multiple exons, including those encoding the Ig-like domains, the transmembrane region and the carboxyl terminus, results in varied isoforms which differ in structure and specificity. Isoform 1 has equal affinity for aFGF and bFGF but does not bind KGF.

Polyclonal

FGFR2

H

IF

All lanes : Anti-FGFR2 Antibody (R22) at 1:1000 dilution
Lane 1: Hela whole cell lysates
Lane 2: K562 whole cell lysates
Lane 3: MCF-7 whole cell lysates
Lane 4: T47D whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 92 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

1:1000

IHC-P

Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term)(Cat#AW5448). AW5448 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

1:25

M

92025

HUMAN

NP_000132.3; NP_001138385.1; NP_001138386.1; NP_001138387.1; NP_001138388.1; NP_001138389.1; NP_001138390.1; NP_001138391.1; NP_075259.4; NP_075418.1