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HIST1H2AG Antibody (Center)

HIST1H2AG Antibody (Center)

ArtNr	ABC-AW5129-U100
Hersteller	Abcepta
Menge	100 ul
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applikationen	WB, FC, IHC-P
Specific against	other
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Similar products	HIST1H2AG, H2AFP, Histone H2A type 1, Histone H2A/p, H2A1
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Primary Accession	P0C0S8
Antigen Type	Synthetic Peptide
Application	IHC-P, FC, WB
Bio Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Bio References	Albig W., et al.Hum. Genet. 101:284-294(1997). Albig W., et al.Biol. Chem. 380:7-18(1999). Dobner T., et al.DNA Seq. 1:409-413(1991). Mannironi C., et al.DNA Cell Biol. 13:161-170(1994). Marzluff W.F., et al.Genomics 80:487-498(2002).
Clonality	Polyclonal
Gene ID	8329; 8330; 8332; 8336; 8969
Gene Name	H2AC11 (HGNC:4737)
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H, M
Legend image 1	Western blot analysis of lysates from mouse NIH/3T3, CEM, Hela, HepG2 cell line (from left to right), using HIST1H2AG Antibody (Center)(Cat. #AW5129). AW5129 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Immunohistochemical analysis of paraffin-embedded M. stomach section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 2	IHC-P
Dilution image 2	1:25
Legend image 3	Immunohistochemical analysis of paraffin-embedded R. stomach section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 3	IHC-P
Dilution image 3	1:25
Legend image 4	Immunohistochemical analysis of paraffin-embedded M. testis section using H. stomach Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 4	IHC-P
Dilution image 4	1:25
Legend image 5	Immunohistochemical analysis of paraffin-embedded M. pancreas section using HIST1H2AG Antibody (Center)(Cat#AW5129). AW5129 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Type image 5	IHC-P
Dilution image 5	1:25
Legend image 6	Flow cytometric analysis of Hela cells using HIST1H2AG Antibody (Center)(green, Cat#AW5129) compared to an isotype control of rabbit IgG(blue). AW5129 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
Type image 6	FC
Dilution image 6	1:25
Other Accession	P84051; P27661; P16104; Q7ZUY3; A9UMV8; Q8R1M2; Q4R3X5; Q9BTM1; P70082; Q3ZBX9; Q00728; P02263; Q4FZT6; Q8BFU2; Q7L7L0; P35062; P04912; Q64523; Q16777; A1A4R1; Q64522; Q8IUE6; P0CC09; Q6GSS7; Q6FI13; P04911; P06897; P02262; P22752; P0C0S9; Q8CGP7; Q99878;
Predicted	Rat, B, X, Y, C, Pr, Zf, D
Isotype	Rabbit Ig
Calculated MW	14091
Antigen Region	63-87
Antigen Source	HUMAN
Target/Specificity	This HIST1H2AG antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 63-87 amino acids from the Central region of human HIST1H2AG.

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ABC-AW5129-U100-datasheet.pdf

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Menge: 100 ul

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Lieferung vsl. bis 06.09.2024

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