ArtNr |
ABC-AT1709a |
Hersteller |
Abcepta
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Applikationen |
WB, ELISA |
Clon |
1G3 |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CD55, CR, DAF, Complement decay-accelerating factor |
Lieferbar |
|
Primary Accession |
P08174 |
Antigen Type |
Recombinant Protein |
Application |
WB, E |
Bio Background |
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. |
Bio References |
1.A novel form of Total Internal Reflection Fluorescence Microscopy (LG-TIRFM) reveals different and independent lipid raft domains in living cells.Asanov A, Zepeda A, Vaca L.Biochim Biophys Acta. 2009 Oct 17. [Epub ahead of print] |
Clonality |
Monoclonal |
Gene ID |
1604 |
Gene Name |
CD55 |
Subtitle |
Mouse monoclonal antibody raised against a partial recombinant DAF. |
Reactivity |
H |
Legend image 1 |
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) . |
Type image 1 |
WB |
Dilution image 1 |
1:500~1000 |
Legend image 2 |
DAF monoclonal antibody (M01), clone 1G3 Western Blot analysis of DAF expression in HeLa ( (Cat # AT1709a ) |
Type image 2 |
WB |
Dilution image 2 |
1:500~1000 |
Legend image 3 |
Western Blot analysis of CD55 expression in transfected 293T cell line by DAF monoclonal antibody (M01), clone 1G3.
Lane 1: CD55 transfected lysate(41.4 KDa). Lane 2: Non-transfected lysate. |
Type image 3 |
WB |
Dilution image 3 |
1:500~1000 |
Legend image 4 |
Detection limit for recombinant GST tagged DAF is approximately 0.03ng/ml as a capture antibody. |
Type image 4 |
E |
Legend image 5 |
Proximity Ligation Analysis of protein-protein interactions between LCK and CD55. HeLa cells were stained with anti-LCK rabbit purified polyclonal 1:1200 and anti-CD55 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). |
Other Accession |
NM_000574 |
Isotype |
IgG1 Kappa |
Antigen Source |
Human: ~~ Positive |
Target/Specificity |
DAF (NP_000565, 35 a.a. ~ 134 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
NCBI Accession |
NP_000565.1; NP_001108224.1 |
|
Clone: 1G3 |
Org Accession |
NP_000565 |
Gene Description |
CD55 molecule, decay accelerating factor for complement (Cromer blood group) |
Gene Summary |
This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq |
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