ArtNr |
ABC-AT1610a |
Hersteller |
Abcepta
|
Menge |
100 ug |
Kategorie |
|
Typ |
Antibody Primary |
Format |
Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Applikationen |
WB, IF, ELISA |
Clon |
6000000 |
Specific against |
other |
Host |
Mouse |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
CPSF3, CPSF73, Cleavage and polyadenylation specificity factor subunit 3, Cleavage and polyadenylation specificity factor 73 kDa subunit, mRNA 3'-end-processing endonuclease CPSF-73, 3127-, CPSF 73 kDa subunit |
Lieferbar |
|
Primary Accession |
Q9UKF6 |
Antigen Type |
Recombinant Protein |
Application |
WB, IF, E |
Bio Background |
Most eukaryotic mRNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3-prime end. CPSF3 encodes the 73-kD subunit of the cleavage and polyadenylation specificity factor (CPSF) and is the pre-mRNA 3-prime-end-processing endonuclease (Mandel et al., 2006 [PubMed 17128255]). |
Bio References |
The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors. Rozenblatt-Rosen O, et al. Proc Natl Acad Sci U S A, 2009 Jan 20. PMID 19136632.Studies of the 5' exonuclease and endonuclease activities of CPSF-73 in histone pre-mRNA processing. Yang XC, et al. Mol Cell Biol, 2009 Jan. PMID 18955505.CSR1 induces cell death through inactivation of CPSF3. Zhu ZH, et al. Oncogene, 2009 Jan 8. PMID 18806823.Conserved motifs in both CPSF73 and CPSF100 are required to assemble the active endonuclease for histone mRNA 3'-end maturation. Kolev NG, et al. EMBO Rep, 2008 Oct. PMID 18688255.The 73 kDa subunit of the CPSF complex binds to the HIV-1 LTR promoter and functions as a negative regulatory factor that is inhibited by the HIV-1 Tat protein. de la Vega L, et al. J Mol Biol, 2007 Sep 14. PMID 17669424. |
Clonality |
Monoclonal |
Gene ID |
51692 |
Gene Name |
CPSF3 |
Subtitle |
Mouse monoclonal antibody raised against a partial recombinant CPSF3. |
Reactivity |
H, M, Rat |
Legend image 1 |
Immunofluorescence of monoclonal antibody to CPSF3 on HeLa cell. [antibody concentration 10 ug/ml] |
Type image 1 |
IF |
Legend image 2 |
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.74 KDa) . |
Type image 2 |
WB |
Dilution image 2 |
1:500~1000 |
Legend image 3 |
CPSF3 monoclonal antibody (M01), clone 6E6. Western Blot analysis of CPSF3 expression in PC-12((Cat # AT1610a ) |
Type image 3 |
WB |
Dilution image 3 |
1:500~1000 |
Legend image 4 |
CPSF3 monoclonal antibody (M01), clone 6E6 Western Blot analysis of CPSF3 expression in Hela S3 NE ( (Cat # AT1610a ) |
Type image 4 |
WB |
Dilution image 4 |
1:500~1000 |
Legend image 5 |
CPSF3 monoclonal antibody (M01), clone 6E6. Western Blot analysis of CPSF3 expression in Raw 264.7((Cat # AT1610a ) |
Type image 5 |
WB |
Dilution image 5 |
1:500~1000 |
Legend image 6 |
CPSF3 monoclonal antibody (M01), clone 6E6. Western Blot analysis of CPSF3 expression in NIH/3T3((Cat # AT1610a ) |
Type image 6 |
WB |
Dilution image 6 |
1:500~1000 |
Legend image 7 |
Western Blot analysis of CPSF3 expression in transfected 293T cell line by CPSF3 monoclonal antibody (M01), clone 6E6.
Lane 1: CPSF3 transfected lysate(77.5 KDa). Lane 2: Non-transfected lysate. |
Type image 7 |
WB |
Dilution image 7 |
1:500~1000 |
Other Accession |
NM_016207 |
Isotype |
IgG2a Kappa |
Antigen Source |
Human: ~~ Positive |
Target/Specificity |
CPSF3 (NP_057291, 585 a.a. ~ 684 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
NCBI Accession |
NP_057291.1 |
|
Clone: 6000000 |
Legend image 8 |
Detection limit for recombinant GST tagged CPSF3 is approximately 0.1ng/ml as a capture antibody. |
Org Accession |
NP_057291 |
Gene Description |
cleavage and polyadenylation specific factor 3, 73kDa |
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