Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Secondary |
Specific against |
other |
Menge |
20 mg |
Host |
Goat |
ArtNr |
ABC-ASR3396 |
Konjugat/Tag |
HRP |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Bio Background |
Anti-Mouse IgG F(c) antibody generated in goat detects specifically mouse IgG. This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. |
Clonality |
Polyclonal |
Subtitle |
Goat Polyclonal, Peroxidase (Horseradish) |
Reactivity |
M |
Immunogen |
Anti-Mouse IgG F(c) was produced by repeated immunization with mouse IgG F(c) fragment in goat. |
Concentration (mg/ml) |
10.0 mg/mL by UV absorbance at 280 nm |
Conjugate |
Peroxidase (Horseradish) |
Application Note |
ELISA 1:10, 000-1:50, 000; Western Blot 1:1, 000-1:10, 000; Immunohistochemistry 1:500-1:2, 500 |
Buffer |
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
Target Species |
Mouse |
Physical State |
Lyophilized |
Target Isotype |
IgG F(c) |
Host Isotype |
IgG |
Reconstitution Volume |
2.0 mL |
Reconstitution Buffer |
Restore with deionized water (or equivalent) |
Stabilizer |
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
Shipping Condition |
Ambient |
Purity |
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab' )2. |
Storage Condition |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
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