Anti-Goat IgG (H&L) (ATTO 425 Conjugated) Pre-Adsorbed Secondary Antibody

Polyclonal

ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are:
A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody.
B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red).
C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue).
D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

>1:5, 000

IF

Goat IgG whole molecule

ATTO 425

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Goat

IgG (H&L)

500 µL

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Ambient

Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.