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F(ab')2 Anti-Rat IgG F(c) (Rhodamine Conjugated) Pre-Adsorbed Secondary Antibody

ArtNr ABC-ASR2872
Hersteller Abcepta
Menge 1 mg
Kategorie
Typ Antibody Secondary
Specific against other
Host Goat
Konjugat/Tag Rhodamine
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Bio Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Clonality
Polyclonal
Subtitle
Goat Polyclonal, Rhodamine (TRITC)
Immunogen
Rat IgG F(c) fragment
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Rhodamine (TRITC)
Application Note
FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
FP Value
4.0 moles Rhodamine (TRITC) per mole of IgG F(ab' )2
Target Species
Rat
Physical State
Lyophilized
Target Isotype
IgG F(c)
Host Isotype
IgG F(ab')2
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Sodium Azide
Shipping Condition
Ambient
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab’)2  or Bovine, Horse and Human Serum Proteins.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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