Vergleich

Mouse IgG F(ab')2 Fluorescein

Hersteller Abcepta
Kategorie
Typ Antibody Primary
Specific against other
Menge 1 mg
ArtNr ABC-ASR2136
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Bio Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Legend image 1
SDS-PAGE of Mouse IgG F(ab')2 Fragment Fluorescein Conjugated . Lane 1: 5 µL Opal Prestained Marker . Lane 2: Reduced Mouse IgG Whole Molecule . Lane 3: Reduced Mouse F(c) Fragment . Lane 4: Reduced Mouse F(ab')2 Fragment Fluorescein Conjugated . Lane 5: Mouse IgM Kappa Myeloma Protein . Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab')2 at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Fluorescein (FITC)
Buffer
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
FP Value
2.5 moles Fluorescein (FITC) per mole of Mouse IgG F(ab' )2
Physical State
Lyophilized
Host Isotype
IgG F(ab')2
Species of Origin
Mouse
PSC Type
Protein
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Thimerosal
Shipping Condition
Ambient
Purity
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by pepsin digestion and extensive dialysis against the buffer stated above.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum.  No reaction was observed against anti-Mouse IgG F(c) or anti-Pepsin.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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