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Anti-Armenian Hamster IgG (H&L) (Fluorescein Conjugated) Secondary Antibody

Hersteller Abcepta
Kategorie
Typ Antibody Secondary
Specific against other
Menge 1 mg
Host Goat
ArtNr ABC-ASR1960
Konjugat/Tag FITC
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Bio Background
FITC Anti-Hamster IgG Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Clonality
Polyclonal
Subtitle
Goat Polyclonal, Fluorescein (FITC)
Reactivity
Ha
Legend image 1
Western Blot of Fluorescein conjugated Goat anti-Armenian Hamster IgG secondary antibody.
Lane 1: Armenian Hamster IgG.
Lane 2: none.
Load: 50 ng per lane.
Primary antibody: none.
Secondary antibody: Fluorescein goat secondary antibody at 1:1, 000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Armenian Hamster IgG.
Other band(s): none.
Type image 1
WB
Immunogen
Armenian Hamster IgG whole molecule
Concentration (mg/ml)
1.0 mg/mL by UV absorbance at 280 nm
Conjugate
Fluorescein (FITC)
Application Note
FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500;
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
FP Value
2.8 moles Fluorescein (FITC) per mole of IgG
Target Species
Armenian Hamster
Physical State
Lyophilized
Target Isotype
IgG (H&L)
Host Isotype
IgG
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Sodium Azide
Shipping Condition
Ambient
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Armenian Hamster IgG coupled to agarose beads.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Armenian Hamster IgG and Armenian Hamster Serum.  Minimal reactivity will occur against Golden Syrian Hamster IgG.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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