Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Secondary |
Specific against |
other |
Menge |
2 mg |
Host |
Goat |
ArtNr |
ABC-ASR1799 |
Konjugat/Tag |
FITC |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Bio Background |
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. |
Clonality |
Polyclonal |
Subtitle |
Goat Polyclonal, Fluorescein (FITC) |
Legend image 1 |
Western blot of Fluorescein conjugated Goat Anti-Mouse IgG F(c) secondary antibody.Lane 1: Mouse IgG F(c).Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Fluorescein goat secondary antibody at 1:1, 000 for 60 min at RT.Blocking: MB-070 for 30 min at RT.Predicted/Observed size: 28 kDa, 28 kDa for Mouse IgG F(c).Other band(s): None. |
Type image 1 |
WB |
Immunogen |
Mouse IgG F(c) fragment |
Concentration (mg/ml) |
2.0 mg/mL by UV absorbance at 280 nm |
Conjugate |
Fluorescein (FITC) |
Application Note |
FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500 |
Buffer |
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
FP Value |
3.1 moles Fluorescein (FITC) per mole of IgG |
Target Species |
Mouse |
Physical State |
Lyophilized |
Target Isotype |
IgG F(c) |
Host Isotype |
IgG |
Reconstitution Volume |
1.0 mL |
Reconstitution Buffer |
Restore with deionized water (or equivalent) |
Stabilizer |
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative |
0.01% (w/v) Thimerosal |
Shipping Condition |
Ambient |
Purity |
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab’)2. |
Storage Condition |
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
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