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Anti-Sheep IgG (H&L) (Texas Red™ Conjugated) Secondary Antibody

ArtNr ABC-ASR1671
Hersteller Abcepta
Menge 2 mg
Kategorie
Typ Antibody Secondary
Specific against other
Host Donkey
Konjugat/Tag Texas Red
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Bio Background
Sheep IgG (H&L) Antibody Texas Red™ Conjugated antibodies are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Clonality
Polyclonal
Subtitle
Donkey Polyclonal, Texas Red®
Legend image 1
Western Blot of Donkey anti-Sheep IgG Texas Red Conjugated Antibody.
Lane 1: Sheep IgG.
Lane 2: None.
Load: 50 ng per lane.
Primary antibody: None.
Secondary antibody: Texas Red donkey secondary antibody at 1:1, 000 for 60 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 28 & 55 kDa, 28 & 55 kDa for Sheep IgG.
Other band(s): None.
Type image 1
WB
Immunogen
Sheep IgG whole molecule
Concentration (mg/ml)
2.0 mg/mL by UV absorbance at 280 nm
Conjugate
Texas Red®
Application Note
FLISA 1:10, 000-1:50, 000; IF Microscopy 1:1, 000-1:5, 000; FlowCytometry 1:500-1:2, 500
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
FP Value
4.7 moles Texas Red® per mole of IgG
Target Species
Sheep
Physical State
Lyophilized
Target Isotype
IgG (H&L)
Host Isotype
IgG
Reconstitution Volume
1.0 mL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% (w/v) Sodium Azide
Shipping Condition
Ambient
Purity
Sheep IgG (H&L) Antibody Texas Red™ Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 2 mg
Lieferbar: In stock
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