Anti-NEDD1 Secondary Antibody

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Microtubules are polymers of tubulin, which exist as heterodimers of alpha-tubulin and beta-tubulin. NEDD1 (neural precursor expressed, developmentally down-regulated protein1; also called GCP-WD) is a centrosomal protein that in mammals associates with the gamma-tubulin ring complex (?-TuRC). ?-TuRC is critical for initiation, or nucleation, of the microtubule assembly. In association with this complex, NEDD1 plays an important role in targeting the ?-TuRC complex to the site of microtubule nucleation and to the mitotic spindle. These events are essential for proper bipolar spindle formation and mitotic progression. Given the casual link between improper spindle function and tumorigenesis, characterization of Nedd1 function will be important to better understand various mechanisms underlying mitotic regulation, chromosome segregation, and cancer development.

Rabbit Polyclonal, Unconjugated

Anti-NEDD1 in Western Blot using Abcepta Immunochemicals' Anti-NEDD1 Antibody shows detection of a 73 kDa band corresponding to endogenous NEDD1 in lysates of S phase HeLa cells silenced for either control Luciferase or NEDD1. In right lane (NEDD1i): lysates from sh-NEDD1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-NEDD1 Antibody was used at 1:10, 000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.

1:10, 000

40 mg/mL by Refractometry

ELISA 1:100, 000; Western Blot 1:10, 000

Human

Antiserum

0.01% (w/v) Sodium Azide

This product was adsorbed against GST from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with endogenous Nedd1 protein. A BLAST analysis was used to suggest reactivity with Nedd1 from human, chimpanzee, macaque, marmoset, cattle, rat, and mouse based on a 100% homology with the immunizing sequence. Expect partial reactivity with Nedd1 from turkey, chicken, salmon, and Danio based on a 91% homology with the immunizing sequence. Cross-reactivity with Nedd1 from other sources has not been determined.