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Cyclooxygenase 1 Antibody

Cyclooxygenase 1 Antibody

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Format	ATTO 655
Menge	Inquiry
ArtNr	ABC-ASM10561-A655-100
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	P23219
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (ASM10561). Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (ASM10561) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Microsome Membrane, Endoplasmic Reticulum Membrane, Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cyclooxygenase 1 Antibody. (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (ASM10561). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (ASM10561) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western blot analysis of Mouse Kidney cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (ASM10561). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Kidney cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (ASM10561) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Western blot analysis of Rat Brain cell lysates showing detection of 68.7 kDa Cyclooxygenase 1 protein using Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (ASM10561). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Cyclooxygenase 1 Polyclonal Antibody (ASM10561) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 68.7 kDa.
Type image 4	WB
Dilution image 4	1:1000
Other Accession	NP_000953.2
Target/Specificity	Detects ~75 kDa.
Immunogen	Synthetic peptide of Human Cyclooxygenase
Purification	Peptide Affinity Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10561-A655-100-datasheet.pdf

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Menge: Inquiry

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Lieferung vsl. bis 06.09.2024

Express-Lieferung: 28 Tage sparen

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