Vergleich

Beclin 2 Antibody

Hersteller Abcepta
Kategorie
Typ Antibody Primary
Specific against other
Format ATTO 594
Menge Inquiry
ArtNr ABC-ASM10493-A594-100
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Primary Accession
P0DM65
Reactivity
H, M
Legend image 1
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite.
Type image 1
ICC/IF
Dilution image 1
1:100
Legend image 2
Western blot analysis of Human HeLa and 293Trap cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Western blot analysis of Mouse Brain cell lysates showing detection of 48.1 kDa Beclin 2 protein using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain cell lysates. Load: 15 µg . Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10493) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 48.1 kDa.
Type image 3
WB
Dilution image 3
1:1000
Other Accession
NP_001277621.1
Target/Specificity
Detects ~48 kDa.
Immunogen
Synthetic peptide from the C-terminal of Mouse Beclin 2 (aa. 410-421)
Purification
Peptide Affinity Purified
Reconstitution & Storage
-20ºC

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: Inquiry
Lieferbar: In stock
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