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Beclin 2 Antibody

ArtNr ABC-ASM10491-100
Hersteller Abcepta
Menge Inquiry
Kategorie
Typ Antibody Primary
Format Unconjugated
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Primary Accession
A8MW95
Reactivity
H
Legend image 1
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10491). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Beclin 2 Polyclonal Antibody (ASM10491) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Beclin 2 Antibody (D) Composite.
Type image 1
ICC/IF
Dilution image 1
1:100
Legend image 2
Western blot analysis of Human 293Trap cell lysates showing detection of ~48.1 kDa Beclin2 protein using Rabbit Anti-Beclin2 Polyclonal Antibody (ASM10491). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Beclin2 Polyclonal Antibody (ASM10491) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~48.1 kDa.
Type image 2
WB
Dilution image 2
1:1000
Legend image 3
Western blot analysis of Rat Liver showing detection of ~48kDa Beclin-2 protein using Rabbit Anti-Beclin-2 Polyclonal Antibody (ASM10491). Lane 1: MW Ladder. Lane 2: Rat Liver (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin-2 Polyclonal Antibody (ASM10491) at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~48kDa.
Type image 3
WB
Dilution image 3
1:1000
Other Accession
NP_001277622.1
Target/Specificity
Predicted molecular weight at ~48.1kDa. Observed bands in the 50-55kDa range, varied by lysates.
Immunogen
Synthetic peptide from the mid-protein of human Beclin 2
Purification
Peptide Affinity Purified
Reconstitution & Storage
-20ºC

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: Inquiry
Lieferbar: In stock
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