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RAS Antibody

RAS Antibody

ArtNr	ABC-ASM10429-100
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	Unconjugated
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
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Primary Accession	B2KGV5
Reactivity	H, M, Rat, B
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:200 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:200
Legend image 2	Immunohistochemistry analysis using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Basal cell of the epidermis.
Type image 2	IHC
Dilution image 2	1:100
Legend image 3	Western blot analysis of Mouse Brain Cortex cell lysates showing detection of ~21 kDa RAS protein using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain Cortex cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~21 kDa.
Type image 3	WB
Dilution image 3	1:1000
Legend image 4	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit at 1:50 for 1-2 hours at RT in dark. Localization: Cell-cell junction.
Type image 4	ICC/IF
Dilution image 4	1:200
Legend image 5	Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-RAS Polyclonal Antibody (ASM10429). Tissue: HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-RAS Polyclonal Antibody (ASM10429) at 1:200 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Lipid-anchor. Focal adhesion. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-RAS Antibody. (C) Composite.
Type image 5	ICC/IF
Dilution image 5	1:200
Other Accession	NP_035067.2
Target/Specificity	Detects ~21kDa.
Immunogen	Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10429-100-datasheet.pdf

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