Dityrosine Antibody

Western Blot analysis of Dityrosine-BSA Conjugate showing detection of 67 kDa Dityrosine-BSA using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 7D4 (ASM10348). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: 3, 5-Dibromotyrosine-BSA. Lane 4: Dityrosine-BSA. Lane 5: Bromotyrosine-BSA. Lane 6: 7-ketocholesterol-BSA. Load: 1 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody (ASM10348) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

1:1000

Specific for dityrosine modified proteins. Does not cross-react with 3, 5-dibromotyrosine or bromotyrosine modified proteins.

Protein G Purified