Legend image 1 |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9 (ASM10336). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody (ASM10336) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 µM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) Hexanoyl-Lysine adduct Antibody. (G, H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria. |
Target/Specificity |
Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, Malondialdehyde, or Methylglyoxal modified proteins. |