Dibromo-tyrosine Antibody

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dibromo-tyrosine Monoclonal Antibody, Clone 6G3 (ASM10334). Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dibromo-tyrosine Monoclonal Antibody (ASM10334) at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A, C, E, G) - Untreated. (B, D, F, H) - Cells cultured overnight with 50 µM H2O2. (A, B) DAPI (blue) nuclear stain. (C, D) Phalloidin Alex Fluor 633 F-Actin stain. (E, F) Dibromo-tyrosine Antibody. (G, H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

1:50

WB

Western Blot analysis of Human Cervical Cancer cell line (HeLa) showing detection of 3-5-Dibromotyrosine-BSA using Mouse Anti-3-5-Dibromotyrosine Monoclonal Antibody, Clone 6G3 (ASM10334). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysate. Lane 3: H2O2 treated HeLa cell lysate. Load: 12 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-3-5-Dibromotyrosine Monoclonal Antibody (ASM10334) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT.

IgG1

Synthetic 3, 5-Dibromotyrosine conjugated to Keyhole Limpet Hemocyanin (KLH).

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