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GABA-A Receptor Alpha 2 Antibody

GABA-A Receptor Alpha 2 Antibody

ArtNr	ABC-ASM10318-RPE-100
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	RPE
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	P23576
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody, Clone S399-19 (ASM10318). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha2 Monoclonal Antibody (ASM10318) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABA-A Receptor Alpha2 Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Mouse Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19 (ASM10318). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 µg. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody (ASM10318) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~55 kDa. Other Band(s): ~ 37 kDa, ~50 kDa, ~70 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Western Blot analysis of Rat Brain showing detection of ~55 kDa GABA A Receptor Alpha 2 protein using Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody, Clone S399-19 (ASM10318). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 10 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-GABA A Receptor Alpha 2 Monoclonal Antibody (ASM10318) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~55 kDa.
Type image 3	WB
Other Accession	NP_001129251.1
Isotype	IgG1
Target/Specificity	Detects ~55kDa. Does not cross-react with GABA-A Receptor Alpha1.
Immunogen	Fusion protein amino acids 350-385 (Cytoplasmic C-terminus) of rat GABA-A Receptor Alpha2
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10318-RPE-100-datasheet.pdf

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