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TCP1-alpha Antibody

TCP1-alpha Antibody

ArtNr	ABC-ASM10311-PCP-100
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	PerCP
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	P11983
Reactivity	H, M, Rat, Rb, Ha, Pr, Pig, Y, B, E, Gp, Dog, D
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a (ASM10311). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody (ASM10311) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Human A431 and HEK293 cell lysates showing detection of TCP1 alpha protein using Rat Anti-TCP1 alpha Monoclonal Antibody, Clone 91a (ASM10311). Primary Antibody: Rat Anti-TCP1 alpha Monoclonal Antibody (ASM10311) at 1:1000.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunocytochemistry/Immunofluorescence analysis using Rat Anti-TCP1-alpha Monoclonal Antibody, Clone 91a (ASM10311). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-TCP1-alpha Monoclonal Antibody (ASM10311) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Centrosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-TCP1-alpha Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Type image 3	ICC/IF
Dilution image 3	1:100
Other Accession	NP_038714.2
Isotype	IgG2a
Target/Specificity	Detects ~60kDa. Also detects ~92kDa. Cross reactivity with human HSP60 has been observed with this antibody in immunoblot analysis. Reacts weakly with Saccharomyces cerevisiae, consistent with the epitope sequence being AKLRS (instead of AKLRA). In C. elegans, it reacts with TCP1 alpha and another CCT subunit. In plants, it recognizes TCP1 of Pisum sativum, and the sequence of Arabidopsis thalania TCP1 over the region of the epitope AKLRA. It has also been shown that it reacts with a subunit of a specialized chaperonin which folds phytochrome.
Immunogen	Recombinant Mouse TCP1 alpha protein fragment (carboxy terminal region).
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10311-PCP-100-datasheet.pdf

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