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GRK (pan) Antibody

GRK (pan) Antibody

Hersteller	Abcepta
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Specific against	other
Format	APC
Menge	Inquiry
ArtNr	ABC-ASM10283-APC-100
eClass 6.1	32160702
eClass 9.0	32160702
Lieferbar
Primary Accession	Q8VEB1
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20 (ASM10283). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody (ASM10283) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GRK (pan) Antibody (D) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Monkey COS cells transfected with untagged GRK6 showing detection of ~65 kDa GRK (pan) protein using Mouse Anti-GRK (pan) Monoclonal Antibody, Clone S145-20 (ASM10283). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with untagged GRK6. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-GRK (pan) Monoclonal Antibody (ASM10283) at 1:200 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~65 kDa.
Type image 2	WB
Dilution image 2	1:1000
Other Accession	NP_061357.3
Isotype	IgG1
Target/Specificity	Detects 70kDa. Cross-reacts with GRK6.
Immunogen	Fusion protein amino acids 381-590 (C-terminus) of mouse GRK5. 91% identical to human, and 99% identical to rat. >55% identity with GRK6 and GRK4. >40% identity with GRK7 and RK.
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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ABC-ASM10283-APC-100-datasheet.pdf

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Menge: Inquiry

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Lieferung vsl. bis 06.09.2024

Express-Lieferung: 28 Tage sparen

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