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HSP47 Antibody

HSP47 Antibody

ArtNr	ABC-ASM10150-A655-200
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	ATTO 655
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	P50454.2
Reactivity	H
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 (ASM10150). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody (ASM10150) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Western Blot analysis of Human Epithelial cell (A431) lysates showing detection of ~47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 (ASM10150). Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody (ASM10150) at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: ~47 kDa.
Type image 2	WB
Dilution image 2	1:1000
Legend image 3	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 (ASM10150). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody (ASM10150) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp47 Antibody. (C) Composite.
Type image 3	ICC/IF
Dilution image 3	1:100
Other Accession	NP_001193943
Isotype	IgG1 Kappa
Target/Specificity	Detects 47kDa.
Immunogen	Human HSP47, full length
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10150-A655-200-datasheet.pdf

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