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HSP90 (total) Antibody

HSP90 (total) Antibody

ArtNr	ABC-ASM10069-A565-200
Hersteller	Abcepta
Menge	Inquiry
Kategorie	Antibodies Primary Antibodies
Typ	Antibody Primary
Format	ATTO 565
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Lieferbar
Primary Accession	P07900
Reactivity	H, M, Rat
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (ASM10069). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10069) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Type image 1	ICC/IF
Dilution image 1	1:100
Legend image 2	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (ASM10069). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10069) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.
Type image 2	IHC
Dilution image 2	1:100
Legend image 3	Western Blot analysis of Rat tissue lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (ASM10069). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10069) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Type image 3	WB
Legend image 4	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (ASM10069). Tissue: muscle tissue. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10069) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Type image 4	IHC
Dilution image 4	1:100
Legend image 5	Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone 4F3.E8 (ASM10069). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (ASM10069) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.
Type image 5	IHC
Dilution image 5	1:100
Other Accession	NP_001017963.2
Isotype	IgG2a
Target/Specificity	Detects ~90kDa. This antibody detects both α and β forms of HSP90 equally well.
Immunogen	Recombinant Human HSP90 purified from E.coli
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

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ABC-ASM10069-A565-200-datasheet.pdf

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Lieferung vsl. bis 06.09.2024

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