ArtNr |
ABC-ASM10016-APC-200 |
Hersteller |
Abcepta
|
Menge |
Inquiry |
Kategorie |
|
Typ |
Antibody Primary |
Format |
APC |
Specific against |
other |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Lieferbar |
|
Primary Accession |
P10809 |
Reactivity |
H, M, Rat, Rb, Ha, Pr, Pig, C, X, B, Sh, Gp, Dog, D |
Legend image 1 |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (ASM10016). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20°C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining. |
Type image 1 |
ICC/IF |
Dilution image 1 |
1:100 |
Legend image 2 |
Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (ASM10016). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. |
Type image 2 |
IHC |
Dilution image 2 |
1:100 |
Legend image 3 |
Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (ASM10016). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. |
Type image 3 |
WB |
Legend image 4 |
Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (ASM10016). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis. |
Type image 4 |
IHC |
Dilution image 4 |
1:100 |
Legend image 5 |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK1, (ASM10016). Tissue: skin Fibroblasts. Species: Human. Fixation: Cold 100% methanol for 30 minutes at -20°C . Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (ASM10016) at 1:1000 for 1 hour at RT. Secondary Antibody: DAKO LSAB2 streptavidin-peroxidase system. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain. Courtesy of: Valentina di Felice, University of Palermo, Italy. |
Type image 5 |
ICC/IF |
Dilution image 5 |
1:100 |
Other Accession |
NP_002147.2 |
Isotype |
IgG1 |
Target/Specificity |
Detects ~60kDa. |
Immunogen |
Recombinant human HSP60 |
Purification |
Protein G Purified |
Reconstitution & Storage |
-20ºC |
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