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SMURF1 Antibody

ArtNr ABC-ASC11844
Hersteller Abcepta
Menge 100 ug
Kategorie
Typ Antibody Primary
Applikationen WB, ELISA, IHC-P
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Primary Accession
Q9HCE7
Application
WB, IHC-P, E
Bio Background
SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1, 2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).
Bio References
Huibregtse JM, Scheffner M, Beaudenon S, et al. A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 1995; 92:2563-7.
Hwang YS, Lee HS, Kamata T, et al. The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1. Genes Dev. 2013; 27:491-503.
Yamashita M, Ying SX, Zhang G, et al. Ubiquitin ligase Smurf1 controls osteoblast activity and bone homeostasis by targeting MEKK2 for degradation. Cell 2005; 121:101-113.
Kwon A, Lee HL, Woo KM, et al. SMURF1 plays a role in EGF-induced breast cancer cell migration and invasion. Mol. Cells 2013; 36:548-55.
Clonality
Polyclonal
Gene ID
57154
Gene Name
SMURF1
Reactivity
H, M, Rat
Legend image 1
Western blot analysis of SMURF1 in 293 cell lysate with SMURF1 antibody at 1 µg/ml.
Type image 1
WB
Legend image 2
Immunohistochemistry of SMURF1 in rat kidney tissue with SMURF1 antibody at 5 µg/ml.
Type image 2
IHC
Other Accession
NP_065162; 31317292
Isotype
IgG
Calculated MW
86114
Antigen Source
homo sapiens
Target/Specificity
SMURF1; SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.
Purification
SMURF1 antibody is affinity chromatography purified via peptide column.
Reconstitution & Storage
SMURF1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
Antibody Form
Liquid
Application Notes
SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 µg/ml. Antibody can also be used for Immunohistochemistry starting at 5 µg/mL.
Positive Control
293 Cell Lysate;
Buffer
SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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