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ERAP1 Antibody

ArtNr ABC-ASC11694
Hersteller Abcepta
Menge 100 ug
Kategorie
Typ Antibody Primary
Applikationen WB, IF, ELISA, IHC-P
Specific against other
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Lieferbar
Primary Accession
Q9NZ08
Application
WB, IHC-P, IF, E
Bio Background
The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1), a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1, 2). It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3). ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3, 4).
Bio References
Hattori A, Kitatani K, Matsumoto H, et al. Characterization of recombinant human adipocytederived leucine aminopeptidase expressed in Chinese hamster ovary cells. J. Biochem. 2000; 128:755-62.
Saric T, Chang SC, Hattori A, et al. An IFN-gamma induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I-presented peptides. Nat. Immunol. 2002; 3:1169-76.
Cui X, Hawari F, Alsaaty S, et al. Identification of ARTS-1 as a novel TNFR1-binding protein that promotes TNFR1 ectodomain shedding. J. Clin. Invest. 2002; 110:515-26.
Akada T, Yamazaki T, Miyashita H, et al. Puromycin insensitive leucyl-specific aminopeptidase (PILSAP) is involved in the activation of endothelial integrins. J. Cell Physiol. 2002; 193: 253-62.
Clonality
Polyclonal
Gene ID
51752
Gene Name
ERAP1
Reactivity
H, M
Legend image 1
Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 µg/ml.
Type image 1
WB
Legend image 2
Immunohistochemistry of ERAP1 in mouse brain tissue with ERAP1 antibody at 5 µg/mL.
Type image 2
IHC
Legend image 3
Immunofluorescence of ERAP1 in mouse brain tissue with ERAP1 antibody at 20 µg/mL.
Type image 3
IF
Other Accession
NP_057526; 94818901
Isotype
IgG
Calculated MW
107235
Antigen Source
homo sapiens
Target/Specificity
ERAP1; ERAP1 antibody is human and mouse reactive. At least two isoforms of ERAP1 are known to exist; this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2.
Purification
ERAP1 Antibody is affinity chromatography purified via peptide column.
Reconstitution & Storage
ERAP1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year.
Antibody Form
Liquid
Application Notes
ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 µg/ml.
Positive Control
;
Buffer
ERAP1 Antibody is supplied in PBS containing 0.02% sodium azide.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100 ug
Lieferbar: In stock
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