ECRG4 Antibody

Q9H1Z8

ECRG4 Antibody: The esophageal cancer-related gene 4 (ECRG4), also known as Augurin, was identified through differential display screening between esophageal cancer and normal tissue. Hypermethylation of CpG sites in the ECRG4 promoter correlate with ECRG4 downregulation in numerous esophageal squamous cell carcinoma tissues.. In transfected cells, overexpression of ECRG4 induced G1 arrest, dephosphorylation of Rb and decreased expression of cyclins D1 and D3. Increased expression of ECRG4 in aged mouse brain is observed compared with young mouse brain. The increased ECRG4 level was accompanied by elevated senescence-associated acidic beta-galactosidase activity, suggesting that ECRG4 is involved in cellular differentiation and senescence.

Polyclonal

ECRG4 (HGNC:24642)

Western blot analysis of ECRG4 in HeLa cell lysate with ECRG4 antibody at (A) 1 and (B) 2 µg/mL.

Immunocytochemistry of ECRG4 in HeLa cells with ECRG4 antibody at 5 µg/mL.

Q9H1Z8; 84417

17183

ECRG4 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of human ECRG4.

The immunogen is located within the last 50 amino acids of ECRG4.

ECRG4 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ECRG4 antibody can be used for detection of ECRG4 by Western blot at 1 - 2 μ g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ g/mL.

ECRG4 Antibody is supplied in PBS containing 0.02% sodium azide.