Hersteller |
Abcepta
|
Kategorie |
|
Typ |
Antibody Primary |
Specific against |
other |
Applikationen |
WB, ELISA |
Menge |
100 ug |
Host |
Rabbit |
ArtNr |
ABC-ASC11154 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Primary Accession |
O75874 |
Application |
WB, E |
Bio Background |
IDH1 Antibody: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Two NADP(+)-dependent isocitrate dehydrogenases have been found as homodimer: IDH1 is predominantly cytosolic and peroxisomal and IDH2 is mitochondrial. The presence of IDH1 in peroxisomes suggests it may play a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic IDH1 serves a significant role in cytoplasmic NADPH production. Defects in IDH1 are involved in the development of glioma. |
Bio References |
Geisbrecht BV and Gould SJ. The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J. Biol. Chem.1999; 274:30527-33.Xu X, Zhao J, Xu Z, et al. Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity. J. Biol. Chem.2004; 279:33946-57.Dang L, White DW, and Gross S. Cancer-associated IDH1 mutations produce 2-hydroxyglutarate. Nature2009; 462:739-44.Smeitink J. Metabolism, gliomas, and IDH1. N. Engl. J. Med.2010; 362:1144-5. |
Clonality |
Polyclonal |
Gene ID |
3417 |
Gene Name |
IDH1 |
Reactivity |
H, M, Rat |
Legend image 1 |
Western blot analysis of IDH1 in HepG2 cell lysate with IDH1 antibody at (A) 1 and (B) 2 µg/mL. |
Type image 1 |
WB |
Other Accession |
NP_005887; 28178825 |
Isotype |
IgG |
Calculated MW |
46659 |
Antigen Source |
Homo sapiens |
Target/Specificity |
IDH1; |
Purification |
IDH1 Antibody is affinity chromatography purified via peptide column. |
Reconstitution & Storage |
IDH1 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Antibody Form |
Liquid |
Application Notes |
IDH1 antibody can be used for detection of IDH1 by Western blot at 1 - 2 µg/mL. |
Positive Control |
HepG2 Cell Lysate; |
Buffer |
IDH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
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